Phis2
Webb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors were transformed into the yeast strain Y187 for subsequent growing on − Trp/−His (−T/−H) medium with different concentrations of 3-AT. Webb24 jan. 2024 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies …
Phis2
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WebbVector Map pGADT7-Rec Vector (Not sold separately) Sold as a part of Cat. Nos. 630490 & 630491 (102016) Page 2 of 4 . Figure 2. pGADT7-Rec Vector cloning site. Webb1 apr. 2024 · To investigate the binding of TaMYB44-D to starch biosynthesis-related genes, the promoter sequences of 12 selected genes were cloned in front of the reporter gene HIS3 to construct the pHIS2.1-Pro plasmids. The plasmids pHIS2.1-Pro and pGADT7-Sec2-TaMYB44-D were co-transfected into the Y187 yeast strain to perform the Y1H …
Webb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors …
WebbCo-transformation of yeast strain Y187 with pGADT7 and pHIS2 . Point-to-point verification. 1) Culture on the corresponding verification plate. 2) Drip X-α-Gal working solution on the … Webb@phis2 a 2x2p2r w f2w 2x2sh. s 2 2 2 x ia 2 fi2 2 fpd2 fq2 2 fr2d2 fs2 2 ftd2 fu2 2 fvf pa x 2 2 2 2 d2 ! 2 #f qa p 2 2 ! 2 ! 2 2 % 2 & f ra 2 %2 2 ( & 2 ...
Webb10 juni 2016 · Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To ...
Webb1 jan. 2016 · This paper reports on research carried out in the field of Human-Computer Interaction (HCI) on the interaction between a human and a mobile device, in… how i met your mother streaming guardaserieWebb10 aug. 2014 · The interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches … high g shape cargo skinny pantsWebbDescription. District Health Information Software 2 Tracker (DHIS2 Tracker) is an open-source, web-based application that supports data collection, and analysis of … high growth tech startupsWebb酵母单杂. 使用pHIS2载体，将启动子序列构建在HIS3报告基因前面的多克隆位点上，对应的转录因子构建在AD载体上即可。. 使用Y187菌株，它相对于AH109菌株在基因组上少 … how i met your mother story lineWebb29 nov. 2024 · Background: Anthocyanins, which belong to flavonoids, are widely colored among red-purple pigments in the Asiatic hybrid lilies (Lilium spp.). Transcription factor … high grundon farm campingWebb27 mars 2015 · To suppress potential background activity, the medium was complemented with 50 or 100 m m 3-amino-1,2,4-triazole (3-AT) and yeast strains harbouring recombinant pHis2.1 were plated on this medium in different dilutions. In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. high grundon cottages and campsiteWebb14 aug. 2015 · 产品报价：询价 更新时间：2015/8/14 10:48:01 产地：广东. 品牌：伟通生物. 型号：各种质粒载体. 厂商性质： 生产型,贸易型,服务型, 公司名称： 深圳市伟通生物科技有限公司 产品关键词： how i met your mother streaming gratuit