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Ghd7-cfp

WebFeb 24, 2024 · Scale bar, 2 mm. (I) Subcellular localization of SID1. Full-length SID1 fused with green fluorescent protein (GFP); the nuclear protein Ghd7 fused with cyan fluorescent protein (CFP) served as a nuclear marker. The two proteins were co-expressed in rice protoplasts. A bright-field image and the merged image are shown on the right. Scale … WebA citrus pulp mastication trait-related ABSCISIC ACID-INSENSITIVE 5-like protein promotes fruit softening via transcriptionally activating pectate lyase gene - ScienceDirect Scientia Horticulturae Volume 314, 15 April 2024, 111933

A bHLH gene NnTT8 of Nelumbo nucifera regulates anthocyanin ...

WebMay 28, 2014 · VirD5 is another A. tumefaciens virulence effector protein and conserved in all strains of A. tumefaciens and A. rhizogenes. It consists of 833 amino acids and contains three putative nuclear... WebJan 31, 2024 · GHD7 and GHD8 are identified with a pleiotropic effect on grain number, plant height and heading date in rice. Both genes delayed flowering through inhibiting the … stanaford medical clinic beckley https://smajanitorial.com

The putative Agrobacterium transcriptional activator‐like virulence ...

WebThe nuclei were identied by co-expressing the nuclear marker Ghd7-CFP with both 35S:SlCOL1-GFP and 35S:GFP. Fluorescence images were acquired using a confocal laser scanning microscope (Leica TCS SP2) after incubating the protoplasts at 28 °C for 12 to 16 h. Representative micrographs are shown. Bars, 7.5 μm (up), 25 μm (down) WebJul 14, 2024 · We hypothesized that Ghd7 is the background determinant affecting the photoperiod sensitivity of Hd1. To confirm this hypothesis, … WebMar 14, 2024 · GHD7, a nuclear protein, was fused to cyan fluorescent protein (CFP) and co-expressed with OsMFT2-GFP in rice protoplasts. OsMFT2-GFP was co-localized with the GHD7-CFP in the nucleus (Figure 2b ), indicating that OsMFT2 is a nuclear protein. Knock-out and overexpressing OsMFT2 altered ABA sensitivity in seed germination and post … stanaford missionary baptist church service

(PDF) The putative Agrobacterium transcriptional activator-like ...

Category:NF-YC12 is a key multi-functional regulator of accumulation of …

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Ghd7-cfp

Subcellular localization of GmFLD and GmLDL2. Empty vector …

WebApr 11, 2024 · For the observation of subcellular localization, the transient expression vectors 35S::NF-YC12-YFP and 35S::NF-YB1-GFP were constructed. 35S::Ghd7-CFP was used as a nuclear localization marker ( Xue et al., 2008 ). Pairs of the constructed vector plasmids were used to co-transform rice protoplast cells. WebSep 20, 2024 · Ghd7 is a well-known protein that regulates heading date and yield in rice, and localizes in the nuclear compartment (Xue et al. 2008 ). Ghd7- cyan fluorescent …

Ghd7-cfp

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WebOct 30, 2024 · When an unrelated nuclear localization marker tagged by CFP (Ghd7-CFP) was used, 71 ± 3% or 84 ± 8% of transformed cells determined by the marker showed … WebGrant Programs Directorate DHS/FEMA. 5071 D Techworld Building. 500 C Street SW. , Washington, District of Columbia 20472 Phone: (1-800) 368-6498. Legal Disclaimer. This …

WebSep 16, 2016 · Ghd7, which is a very important CO-like gene controlling grain number, plant height, and heading date in rice ( Xue et al., 2008 ), also regulates stress tolerance. The … WebSep 1, 2024 · To determine the subcellular localization of AtWRKY47, we co-expressed 35 S:WRKY47-GFP (green fluorescent protein) with the nuclear marker 35 S:Ghd7-CFP (cyan fluorescent protein) in Arabidopsis protoplasts. Nuclear localization of AtWRKY47 was observed since GFP fluorescence completely overlapped with CFP signal (Fig. 2 e).

WebSep 25, 2024 · I actually got 2lbs of it my orginal intent was to test it with my gen5 g17 but the throat is way to short to use the hornady xtp 147gr with that powder. I eventually tried …

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WebOur analysis revealed a regulatory network that may provide an approximation to the genetic constitution of the optimal heterosis observed in this hybrid. In this network, Ghd7 works … stan against evil canceledWebADH7. A gene on chromosome 4q23-q24 that encodes class-IV alcohol dehydrogenase 7 mu or sigma subunit, which like other alcohol dehydrogenases metabolises various … stanaford wv real estateWebAug 10, 2015 · A Ghd7–cyan fluorescent protein (CFP) fusion protein was used as a nuclear localization marker, as Ghd7 is a previously reported nuclear protein in rice (Xue et al., 2008). A SNAC3–GFP fusion protein was co-expressed with Ghd7–CFP in rice protoplasts, and the fluorescence signal was observed and captured using confocal microscopy. persnickety holidayWebNational Center for Biotechnology Information persnickety holiday 2018WebMar 1, 2011 · To assay the sub-cellular localization of GHD8, the coding sequence of GHD8 was fused to yellow fluorescent protein (YFP); in contrast, GHD7, which is a nuclear protein, was fused to cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. persnickety dry cleaning bozemanWebJun 21, 2024 · Ghd7 expression is sensitive to photoperiod. Under LD conditions, Ghd7 expression is induced and thus Ehd1 and Hd3a expression is suppressed. Under SD … persnickety holiday clothingWebThe nuclei were identified by co-expressing the nuclear marker Ghd7-CFP with both 35S:SPL13-GFP and 35S:GFP. Fluorescence images were acquired using a confocal laser scanning microscope (Leica TCS SP2, MRC Centre for Regenerative Medicine, The University of Edinburgh, Edinburgh, UK) after incubating the protoplasts at 28 °C for … persnickety holiday rose tunic